Comic Book Artists Mike Mignola and Tim Bradstreet worked as conceptual artists.
In the scene where Blade and the bloodpack are walking towards the house of pain, you see briefly through Whistler's thermal-scope that Blade is hot blooded unlike the other vampires.
The phrase "a man without fear" is used in this film. This is another comic book reference, this one a reference to Daredevil, which is now a Ben Affleck film.
When Blade returns to his headquarters early in the movie, Scud remarks, "The Dark Knight Returns!" This is a reference to another comic book character that hunts by night, Batman, who was the subject of a classic comic book miniseries.
No real reapers were hurt during the making of this film.
DVD Easter Eggs: (Hidden So You Don't See Anything You Don't Want To See)
Edition: New Line Platinum Series
Description: Special Thanks
Put in disc two. Once the Special Features menu comes up, press the left arrow and this will highlight the New Line symbol, press enter. This will take you to the Blade II Platinum DVD Credits.
From the disc's Main Menu go to the 'Production Workshop' and then select the 'Visual Effects' menu entry. Once you get to that submenu, highlight the 'Progress Reports' menu entry and then press the 'Right' arrow key on your remote control, followed by the 'Up' arrow key. This will highlight a vampire glyph in the upper right hand corner of the screen. Press 'Enter'' now and you will see director Guillermo Del Toro having some fun with an adult toy.